Coding
Part:BBa_K4025001:Design
Designed by: Manini Penikalapati Group: iGEM21_Lubbock_TTU (2021-10-16)
ChiS inter-membrane protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Chis was codon-optimized for E. coli and illegal sites according to Type IIS assembly (RFC[1000]) were removed via mutations of codons in restriction sites.
Source
The source of ChiS is the sequence from GenBank (VC_0622).