Coding

Part:BBa_K4025001:Design

Designed by: Manini Penikalapati   Group: iGEM21_Lubbock_TTU   (2021-10-16)


ChiS inter-membrane protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Chis was codon-optimized for E. coli and illegal sites according to Type IIS assembly (RFC[1000]) were removed via mutations of codons in restriction sites.


Source

The source of ChiS is the sequence from GenBank (VC_0622).

References